GENETICS - BIOL 7        EXAM II - October 13,  2005    NAME:

DO NOT USE ANY OTHER PAPER AT ANY TIME WITHOUT INSTRUCTOR PERMISSION

Part I: [45 points]  Choose the one BEST answer.  Circle its letter. Read each question and all 5 choices carefully because more than one answer might seem correct at first glance.  1.5  points each

1.  In Drosophilla, if a red-eyed female (wild type, + +) is crossed with a white-eyed male (w), in which generation does it become clear that w is sex linked?
          a.  P
          b.  F1
          c.  F2
          d.  not enough information
          e.  this cross can not demonstrate the sex-linkage of w

2.  In humans,
          a.  a man always passes his X chromosome to his daughters
          b.  a man always passes his Y chromosome to his sons
          c.  a woman always passes an X chromosome to her sons
          d.  all of the above
          e.  none of the above are always true

3.  The offspring from a testcross with the parental allelic configuration
          a.  is always the largest group of offspring because this is the wild type
          b.  is half or more of the offspring because when crossing over occurs, only one pair of chromatids are involved, so there are always half that remain parental
          c.  the smallest group are the DCO
          d.  is the largest group because when crossing over occurs, only one pair of chromatids are involved, so there are always half that remain parental
          e.  no generalization can be made because there is so much variety in biology

4.  In moths, the hemigametic sex is ______ ; in Drosophila, the hemigametic sex is ________
          a.  male ..... female
          b.  female .....  male
          c.  female  ..... female
          d.  male .....  male
          e.  there is none ..... male

5. As the distance between two loci increases,  mapping them becomes
          a.  more affected by interference
          b.  less accurate
          c.  easier to calculate
          d.  more accurate
          e.  a and b
          f.  c and d

6.  Examination of test cross data showed that two linked genes, p and q, had a recombinant frequency (RF) of 0.091. A third gene, d, had RFs of 0.173 with p, and 0.256 with q. What is the order of these genes?
        a.  p-q-d
        b.  q-p-d
        c.  q-d-p
       d.  the genes are not all linked to each other
       e.  it is not possible to be sure of the order from these data

7.  Women who are heterozygous for an X-linked condition causing the absence of sweat glands exhibit patches of skin with sweat glands and patches lacking sweat glands. This (mosaic) effect is the result of
          a.  incomplete dominance
          b.  X-chromosome inactivation
          c.  complete dominance
          d.  all heterozygotes would be expected to display this phenomenon regardless of sex
          e.  co-dominance

8.   A trihybrid can produce ___ different gametic genotypes (with respect to those three genes under study)
          a.  2
          b.  3
          c.  4
          d.  8
          e.  not enough information given

9.  Is the answer to #8 different depending on whether or not the genes are linked?  explain briefly

10.  A green female was crossed with a yellow male; a crisscross inheritance pattern would be
          a.  all females are green and all males are yellow
          b.  all females are yellow and all males are green
          c.  all offsprings are green because green is dominant
          d.  50% of the offsprings are green, 50% are yellow
          e.  it depends on whether, in this example, the males or females are heterogametic

11.  In humans, the greater the number of supernumerary X chromosomes (e.g., XXX or XXY) results in
          a.  a higher dosage of femininity
          b.  the female being more fertile
          c.  increased retardation
          d.  a and b
          e.  all of the above

12.  A normal vision woman whose father was colorblind (sex linked) has children with a normal vision man.  The chance of their having an affected son is
          a.  0
          b.  25%
          c.  50%
          d.  75%
          e.  100%

13.  The chance of the couple described in #12 having an affected daughter is
          a.  0
          b.  25%
          c.  50%
          d.  75%
          e.  100%

14.  Epistasis is when
          a.  the expression of a genotype at one locus depends on the genotype at another locus
          b.  an allele masks the expression of another allele at the same locus
          c.  the dosage of an allele effects the expression of another allele
          d.  a and b
          e.  all of the above

15.  Wild type alleles are
          a.  always dominant
          b.  always recessive
          c.  either dominant or recessive
          d.  neither dominant nor recessive
          e.  any of the above might be true; it depends on the gene

16.  To determine the distance between three genes, a,b, and c,
          a.  only one dihybrid cross is necessary
          b.  two different dihybrid crosses are necessary: a - b and b - c
          c.  three dihybrid crosses are necessary: a-b, b-c, and a-c
          d.  each of these genes must be mapped with respect to a fourth gene, d
          e.  one cannot map three genes using dihybrid crosses

17.  In X-linked recessive disorders
          a.  more males than females show the phenotype under study
          b.  all of the offspring of an affected male are affected
          c.  all of the sons of an affected male will pass the condition to their offspring
          d.  if the recessive allele is rare, almost all the persons showing the phenotype are female
          e.  only females can transmit the allele; only males can express it

18.  A test cross is a cross in which
          a.  one parent is heterozygous at all loci under consideration
          b.  both parents are heterozygous at all loci under consideration
          c.  one parent is male; genes under consideration are X-linked
          d.  one parent is homozygous recessive (either male or female)
          e.  either c or d may be true for a cross to be used as a test cross

19.  The distance between loci on a chromosome determines the probability of
          a.  mutation
          b.  recombination
          c.  hemizygosity
          d.  gene linkage
          e.  heterozygosity

20.  Genes A, B, D, E are linked in that order on a chromosome.  A-B is 15cM, B-D is 10cM, D-E is 20cM.  The probability of triple cross over among these four genes is
          a.  30%
          b.  3%
          c.  0.3%
          d.  0.03%
          e.  triple crossovers are not detected

21. The term "wild type" indicates
          a.  organisms found outside captivity
          b.  organisms out of control
          c.  organisms particularly prone to mutate
          d.  organisms which breed true
          e. the accepted "standard" strain of an organism, or allele of a gene (genotype or phenotype)

22.  The purpose of a testcross is to determine the
          a.  recombination frequency between or among linked genes
          b.  genotype of the tested parent
          c.  genotypes of the progeny because any dominant phenotype must be represent heterozygosity
          d.  a and b
          e.  all of the above

23.  Genes r and s are linked autosomal genes whose recombinant frequency is 5%.  Genes g and h are X-linked genes, 10 map units apart. A homozygous dominant female is crossed with a recessive male; the F1 females are backcrossed. Which of the following would be expected for the testcross progeny?
          a.  nearly equal frequency of r+sg+h+, r+sgh, rs+g+h+, rs+gh classes
          b.  independent segregation of some alleles with respect to some others
          c.  different phenotypic ratios in males and females
          d.  a and b
          e.  all of the above

24.  For the system in #23 - which of the following would be expected for the testcross progeny  phenotypes?
          a.  r+s & rs+together  ~ 95% ; r+s+ & rs together ~ 5%
          b.  r+s & rs+together  ~ 5% ; r+s+ & rs together ~ 95%
          c.  approximately equal numbers of r+s+, r+s, rs+, rs
          d.  either a or b is true, but we do not know which; both are not true
          e.  the answer is different in males and females
          f.  not enough information given

25.  For the system in #23 - which of the following would be expected for the testcross progeny  phenotypes?
          a.  g+h & gh+together  ~ 90% ; g+h+ & gh together ~ 10%
          b.  g+h & gh+together  ~ 10% ; g+h+ & gh together ~ 90%
          c.  approximately equal numbers of g+h+, g+h, gh+, gh
          d.  either a or b is true, but we do not know which; both are not true
          e.  the answer is different in males and females
          f.  not enough information given

26.  A triply wild-type female mated with a triply recessive mutant male gives rise to a large number of offspring.  All of the offspring are wildtype.  Among the F-2, the males represent many different phenotypes, but the females are all wildtype.  This indicates
          a.  the genes are sex-linked; the mother (P1) was heterozygous at several loci
          b.  the genes are sex-linked; the mother (P1) homozygous dominant
          c.  the genes are sex-linked but this is a ZZ/ZW organism: the female is hemizygous
          d.  the genes are autosomal
          e.  not enough information given

27.  If the genes above are linked, which  mating would allow the best mapping?
          a.  the F-1
          b.  the female P-1 with the F-1 male
          c.  the female F-1 with the P-1 male
          d.  one would have to find another strain altogether - none of these are helpful
          e.  the genes are not linked

28.  The terms “in repulsion” and “coupled” are used to describe chromosomes with alleles of genes (under consideration) that are
          a.  all dominant versus all recessive
          b.  all wild type versus all mutant
          c.  a mix of dominant and recessive versus all dominant or all recessive
          d.  all mutant versus all wild type
          e.  a mix of wild type and mutant versus all wild type or all mutant

29.  In Drosophilla, if a white-eyed female (w, w) is crossed with a red-eyed male (wild type   +), in which generation does it become clear that w is sex linked?
          a.  P
          b.  F1
          c.  F2
          d.  not enough information
          e.  this cross does not demonstrate the sex-linkage of w

30.  If the woman in # 7 had children with a normal (sweaty) male with no family history of this disorder, the chance that a son would lack sweat glands is
          a.  0
          b.  25%
          c.  50%
          d.  75%
          e.  100%

Part II:  [56 points] Answer each question completely and clearly.  Show your work for possible partial credit.  Make sure your answer is clearly stated, and easy to find – perhaps put a box around it.  Make sure you have answered all the parts of the question.

1.   16 points  Do the parts of this problem in order – each answer must make sense given the information you have available up to that point in the story.

          a.  An individual heterozygous at loci A & B was test crossed.  The counted offspring were as follows.  What were the parents’ genotypes and phenotypes, including any linkage information, map distances, etc.  that you can determine (give complete information).

12  AB
36  Ab
34  aB
18   ab

          b.  An individual heterozygous at loci B & D was test crossed.  The counted offspring were as follows.  What were the parents’ genotypes and phenotypes, including any linkage information, map distances, etc.   that you can determine (give complete information).

38  DB
  8  Db
12  dB
42  db

                        c.  If these genes are all in the same species, are A & D linked?  If so, how far apart are they?  Explain (show your work)

d.  If these genes are all in the same species, knowing only what you know so far:
          a.  A & B are coupled, but D is in repulsion to both of them
          b.  B & D are coupled, but A is in repulsion to both of them
          c.  A & D are coupled, but B is in repulsion to both of them
          d.  all 3 genes are coupled
          e.  not enough information give
          f.  genes are not coupled or in repulsion in a species, only in an individual

          e.  An individual heterozygous at loci A & D was test crossed.  The counted offspring were as follows.  What were the parents’ genotypes and phenotypes, including any linkage information, map distances, etc.  that you can determine (give complete information).

32  AD
24  Ad
20  aD
24  ad

          f.  Now it turns out that the parents in part (a), (b), and (e) are the same (as each other).   Taking all of this information into account, what were the parents’ genotypes and phenotypes, including any linkage information, map distances, etc. (give complete information).

          g.  There should be some discrepancies in these answers.  Explain why they appear to contradict each other, and how you resolve the discrepancies.


2.  Solve the following mapping problems (#2 & 3) 15 points each.  Your final answers should include the genotypes and phenotypes of both parents, with the genes arranged in the correct order and in the correct allelic configuration (i.e. dominant and recessive alleles arranged properly on each chromosome).  Map distances  and interference must also be calculated.  Please place the completed answer in one spot so that all of the components can be easily found.
 
+    +    +      23
+    +    d      20
+    b    +    435
a    +    +        2
a    +    d    445
a    b    +       40
a    b    d      32
+    b    d        3


3.                                  ♀           ♂

r          q          g                       27
r          q          +                         1
r          +          g                     389
r          +          +                       57
+          +          +      1010         40
+          q          +                     452
+          q          g                       32
+          +          g                         2

4.  10 points  Solve the following pedigree. For each individual, write the genotype [write it next to the symbol in the pedigree]. Determine the inheritance pattern for the indicated trait (e.g. recessive, dominant, sex-linked, etc.)

Name the propositus:     _______________

Inheritance pattern:        _______________
(explain if necessary)

Is this pedigree consistent with sex-linkage?  Explain.

What data would confirm that it definitively is, or is not, a sex-linked gene?

Why might the propositus come to a counselor?

bonus: Write a question that you studied for, and forgetful me, I neglected to ask.  Answer your question.  PLEASE ask yourself something you can answer well!  PLEASE answer the question you actually ask.  [up to 5 points awarded based on question & answer]