Molecular Biology & Biochemistry - Biol 99

“Portfolio”:  Metabolism

 For all components of the “portfolio” assignment series:
•    hand drawing is required
•    any explanations longer than a sentence must be typed
•    labeling and short explanations may be neatly handwritten
•    everything must be neat and legible
•    drawings should be sufficiently large and spread out that all components are easily distinguished and sufficient detail is included
•    nothing may be cut and pasted from hard copy sources or electronic sources

Metabolism

VIII.  Regulation of the glycolysis pathway                                                                      due 10th class session  [11/10/11]
•    include each step of glycolysis
•    write the molecular form for each component
•    include any molecule entering or leaving the pathway as a reactant or product
•    include one or two-headed arrows designating reversible or “irreversible” reactions, as appropriate
•    highlight (in some fashion) each step in which “high energy”  molecules are consumed or generated
•    for each regulated step, show the reaction that is regulated
•    include any molecule involved in the regulation
•    show which reaction (or component of a reaction) is up- or down-regulated
•    make clear which molecules up- or down-regulate which step (and in which direction)

IX.  Regulation of the Krebs cycle                                                                                  due 12th class session  [11/15/11]
•    include each step of the TCA cycle
•    write the molecular form for each component
•    include any molecule entering or leaving the pathway as a reactant or product
•    include one or two-headed arrows designating reversible or “irreversible” reactions, as appropriate
•    highlight (in some fashion) each step in which “high energy”  molecules are consumed or generated
•    for each regulated step, show the reaction that is regulated
•    include any molecule involved in the regulation
•    show which reaction (or component of a reaction) is up- or down-regulated
•    make clear which molecules up- or down-regulate which step (and in which direction)

X.  Regulation of the electron transport chain and oxidative phosphorylation                      due 13th class session  [11/17/11]
•    include each step
•    include any molecule entering or leaving the pathway as a reactant or product
•    include one or two-headed arrows designating reversible or “irreversible” reactions, as appropriate
•    highlight (in some fashion) each step in which “high energy” molecules are consumed or generated
•    highlight (in some fashion) each step in which protons move across a membrane, and the direction
•    keep track of electrons

XI.  Tracking carbons through glycolysis and the citric acid cycle                                       due 14th class session  [11/17/11]
•    trace the path of each carbon in one glucose molecule until its release as CO2
•    be sure to indicate (label) and track each carbon individually
•    how many “rounds” of the TCA cycle are required until all the carbons of a single glucose molecule are released as CO2 ?  Be sure you can back up your answer

XII.  Other molecules that feed into glycolysis and/or the TCA cycle                                 due 15th class session  [11/22/11]
•    include each step in both pathways
•    include any molecule entering or leaving the pathway as a reactant or product
•    indicate where each amino acid, nitrogenous base, and lipid derivative can enter the pathway

XIII.  Other molecules that derive from glycolysis and/or the TCA cycle                            due 15th class session  [11/22/11]
•    include each step in both pathways
•    include any molecule entering or leaving the pathway as a reactant or product
•    indicate where each amino acid, nitrogenous base, and lipid derivative is derived from the pathway (the exit point)

XIV.  Catabolism of lipids: fatty acids and glycerol   (see XV for bullet list)                        due 19th class session  [11/22/11]
XV.   Anabolism of lipids: fatty acids and glycerol                                                            due 20th class session  [11/22/11]
•    include each step
•    write the molecular form for each component
•    include any molecule entering or leaving the pathway as a reactant or product
•    include one or two-headed arrows designating reversible or “irreversible” reactions, as appropriate
•    highlight (in some fashion) each step in which “high energy”  molecules - and which ones (include high phosphoryl transfer potential and redox types) - are consumed or generated